Cyclic Peptide Inhibitors of the β-Sliding Clamp in Staphylococcus aureus
Figure 3
Identification of peptides that interfere with DnaN-DnaN interaction.
In the reverse two-hybrid strain (SC01) interaction between T18:DnaN and DnaN:T25 resulted in inability to grow in the presence of 5-FOA (A panel; vector). After screening of our SICLOPPS library, growth was restored in 7 clones.A. (B) The cyclic peptides identified in (A) were expressed in the original bacterial two-hybrid strain (BTH101) containing T18:DnaN and DnaN:T25 fusion plasmids and the level of β-galactosidase activity was determined as described in Materials and Methods.