'Special K' and a Loss of Cell-To-Cell Adhesion in Proximal Tubule-Derived Epithelial Cells: Modulation of the Adherens Junction Complex by Ketamine
Figure 4
Ketamine stimulates phosphorylation of ERK and p38 MAPK.
HK2 cells were cultured in 5 mM glucose containing media for 48 hrs prior to overnight serum starvation. Cells were stimulated with Ketamine (1 mg/mL) for 0–30 minutes under serum free conditions and expression of p-ERK and p-P38 assessed by immunoblotting. Upper panels show representative blots for each protein and re-probed for Total ERK and Total p38 respectively as a loading control. Lower panels show mean (±SEM) densitometry data, normalised against the non-stimulated low glucose control (100%), from 3 or more separate experiments. Each lane in the representative blot corresponds to the associated bar in the graph. Key significances are shown, **P<0.01, *** P<0.001.