Deletion of Thioredoxin Reductase and Effects of Selenite and Selenate Toxicity in Caenorhabditis elegans
Figure 3
Effect of Se on C. elegans length and brood size.
(A) Synchronized N2 L1s were inoculated (100/well) into axenic media (0.0001 mM) supplemented with 0 (▴), 0.05(○), or 0.2 (□) mM Se as Na2SeO3. Worm length was measured daily. Solid line shows the regression of 0 and 0.05 mM Se treatments combined, and dashed line shows regression of the 0.2 mM Se treatments. Values represents the mean ± SEM. Two-way ANOVA indicates significant effect of day (P<0.001), Se (P<0.001), and interaction (P<0.001); * indicates a significant difference from 0.2 mM Se treatment, P<0.05. (B) Synchronized N2 L1s were inoculated (2–7/well) into axenic media (0.0001 mM) supplemented with 0, 0.05, or 0.2 mM Se as sodium selenite. Average brood size/adult was assessed in triplicate wells for up to 15 days. Values represents the mean ± SEM (P = 0.686), NS indicates no significant difference. (C) Synchronized N2 L1s were inoculated at two densities, 5 L1/well (•,▪) or 100 L1/well (○,□), into axenic media supplemented with 0 mM Se (•,○) or 0.2 mM Se (▪,□) as selenite. Average offspring/adult was assessed in triplicate at days 6, 9, and 12, and also at day 15 for 5 L1/well worms grown in 0.2 mM Se. Values represents the means ± SEM. A, B, C: Means with different letters are statistically different, P<0.05.