TLR2, TLR4 and the MyD88 Signaling Are Crucial For the In Vivo Generation and the Longevity of Long-Lived Antibody-Secreting Cells
Figure 8
TLR2, TLR4 and MyD88 signaling can sustain ASC B220neg in peritoneal cavity.
A representative dot plot of ASC B220neg (B220negCD138pos from CD43-positive gated cells) analyses is shown. Cells from peritoneum (A, D), spleen (B, E) and bone marrow (C, F) were obtained of TLR4 mutant (left), TLR2 KO and MyD88 KO (right) Natterins immunized mice after 48, 74 and 120 days. The bars representative of the absolute numbers of B220negCD43posCD138pos cells were determined from total mononuclear cells by multiparametric flow cytometer using Rat IgG2ak PerCP-Cy5-anti-mouse CD45R/B220, Rat IgG2ak FITC-anti-mouse CD43, and Rat IgG2ak PE-anti-mouse CD138. *p<0.05 compared to control mice; and #p<0.05 compared to WT mice immunized with Natterins.