Developing Transgenic Jatropha Using the SbNHX1 Gene from an Extreme Halophyte for Cultivation in Saline Wasteland
Figure 2
Genetic transformation of J. curcas via microprojectile bombardment.
(A) Transient gus expression on embryo axis after 24 h of bombardment. Selection of transformants (B) during 1st round (8 days old), (C) during 3rd round (60 days old) on hygromycin. Regeneration of transformants; (D) multiple shoot induction, (E) multiple shoot regeneration (120 days old), (F) rooting (200 days old), (G) hardening of transgenic plant in plastic jar (250 days old). GUS assay of (H) non transformed leaf and (I) leaf of transgenic plant.