Assessments of Total and Viable Escherichia coli O157:H7 on Field and Laboratory Grown Lettuce
Figure 1
Effect of incubation time and PCR primer on detection of live and dead cells by real-time PCR.
Live or isopropanol-inactivated dead cells of E. coli O157:H7 ATCC 700728 (5×107 CFU) were incubated in the dark with or without 40 µM PMA for 15, 30, or 60. Real-time PCR was performed using the lpfA or hlyA primer sets [20], [21]. Each bar represents the mean ± stdev for three independent replicates.