Branched-Chain Amino Acid Supplementation Reduces Oxidative Stress and Prolongs Survival in Rats with Advanced Liver Cirrhosis
Figure 4
Immunohistochemistry of 4-Hne adducts in rats with advanced liver cirrhosis.
A strong intracellular 4-Hne adduct immunostaining (grade 3) is detected in the liver of rats not supplemented with BCAA (Fig. 4-A; Left, magnification×25; Right, magnification×400). A mild intracellular 4-Hne adduct immunostaining (grade 1) is detected in the liver of rats supplemented with BCAA (Fig. 4-B; Left, magnification×25; Right, magnification×400). 4-Hne index is decreased in the BCAA supplementation group (closed columns, n = 6) compared to the control group (open columns, n = 4) (Fig. 4-C). Hepatic 8-OHdG concentration is decreased in the BCAA supplementation group (closed columns, n = 6) compared to the control group (open columns, n = 4) (Fig. 4-D). 4-Hne, 4-hydroxynonenal; BCAA, branched-chain amino acids; 8-OHdG, 8-hydroxy-2′-deoxyguanosine.