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Proline Accumulation in Leaves of Periploca sepium via Both Biosynthesis Up-Regulation and Transport during Recovery from Severe Drought

Figure 3

Stem and root proline biosynthesis metabolism in P. sepium seedlings during drought stress and re-watering.

Two different degrees of drought treatment, −0.4 and −1.0 MPa, were set. Plants were re-watered after being drought-stressed for 96 h under −0.4 MPa or −1.0 MPa. Parameters were determined after 0, 24 and 96 hours of drought stress or re-watering. (A, D) proline concentration in stems and roots; (B, E) activity of Δ1-pyrroline-5-carboxylate synthetase (P5CS) in stems and roots; (C, F) activity of ornithine δ-aminotransferase (OAT) in stems and roots. Data represent the mean ± standard deviation (SD) of three replicates. Different small letters along line of −0.4 MPa and capital letters along line of −1.0 MPa indicate significant differences between different time points at P<0.01, respectively.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0069942.g003