Macromolecular Crowding as a Suppressor of Human IAPP Fibril Formation and Cytotoxicity
Figure 5
Structural differences of hIAPP aggregates upon incubation in crowded environments as detected by AFM.
AFM images showing the structures of 50 µM hIAPP after ∼15 h of incubation in solutions with and without crowding reagents. A hIAPP only. B–E Incubation of hIAPP in 20% or 40% (w/v) of Ficoll, dextran, BSA and lysozyme. For an easier comparison of the hIAPP fibril sizes, insets in the left images of D and E show the same magnification as the other images displaying fibrils. All scale bars correspond to 1 µm. The color scale represents a height of 30 nm for images A–C and 5 nm for images D and E.