Overexpressed DNA Polymerase Iota Regulated by JNK/c-Jun Contributes to Hypermutagenesis in Bladder Cancer
Figure 2
The expression of pol ι is dependent on activated JNK/c-Jun.
(A) The kinetics of pol ι, p-JNK and p-c-Jun expression under UV damage in HEK293 cells. (B) The effect of UV damage on the activity of the POLI promoter region. *Statistically significant difference compared to the pGL3-275/+63 and pGL3–275/+63MU construct (p<0.01; Student's t-test). **Statistically significant difference compared to the cells treated with or without UV (p<0.01). (C) The expression of pol ι, c-Jun and p-c-Jun in RT4 and T24 bladder cancer cells. The expression of pol ι was observed in (D) HEK293, T24 and RT4 cells treated with different concentrations of SP600125 cultured for additional different lengths of time; (E) HEK293 cells treated with PD98059 or SB203580; (F) HEK293 cells treated with or without SP600125 and UV; (G) HEK293 cells transfected with siRNA-c-Jun for 48 hours. The non-specific siRNA was used for control. Densitometric values were indicated below each panel.