In Vitro Induction of Regulatory CD4+CD8α+ T Cells by TGF-β, IL-7 and IFN-γ
Figure 7
Relative Runx3 and ThPOK mRNA expression.
mRNA isolated from FACS-purified CD4+CD8α+ T cells was reverse-transcribed and the cDNA amplified. (Top graph) Runx3 relative expression was normalized using total naïve CD4+ T cell mRNA. (Bottom graph) ThPOK relative expression was normalized using total naïve CD8+ T cell mRNA. (n = 3).