MOR Is Not Enough: Identification of Novel mu-Opioid Receptor Interacting Proteins Using Traditional and Modified Membrane Yeast Two-Hybrid Screens
Figure 1
Mapping the MOR/MORIP interaction.
Candidate MORIPs identified in the traditional and MYTH Y2H screens (preys) were tested for interaction with each of the intracellular loops (IL) and carboxyl-terminus (C-tail) of the mu-opioid receptor (baits) in a directed Y2H assay. An empty bait vector (pACT) was used as a negative control. A positive interaction is indicated by the production of a blue yeast colony in the β-galactosidase assay.