Bone Morphogenetic Proteins Stimulate Mammary Fibroblasts to Promote Mammary Carcinoma Cell Invasion
Figure 4
BMP signaling is altered in Human Breast CAF compared to NAF.
Normal Associated Fibroblasts (NAF) and Carcinoma Associated Fibroblasts (CAF) were derived from human breast tissue RNA was isolated an analyzed by SYBR RT-PCR. A) RT-PCR was performed for known modulators of the BMP pathway. Samples are normalized relative to GAPDH mRNA expression and displayed as fold change. * Indicates statistical significance with a p-value <.01 from a student t-test. N.S. indicates no significance between two groups. B&C) Western blot for BMP response revealed active BMP signaling in NAF and CAF. Cells were serum starved overnight and treated in serum free medium with recombinant human BMP4 (100 ng/ml), Noggin (1 ug/ml) and DMH1 (20 µM). D) RT-PCR for BMP response canonical target gene id1 after 24 hours of treatment with BMP4 (100 ng/ml), Noggin (1 ug/ml) and DMH1 (20 µM) demonstrated unique transcriptional response between NAF and CAF. Error bars indicate standard deviation.