The Requirement of WHIRLY1 for Embryogenesis Is Dependent on Genetic Background in Maize
Figure 4
(A) Southern analysis of individual plants from an emb16 segregating family by using a Mu1/Mu2 specific probe. “++”, WT and “+−”, heterozygote for the emb16 mutation. The genomic DNAs were digested with EcoRI. Arrow points to a 7.5 kb fragment that co-segregates with the emb16 mutation. (B) Gene structure of Why1 gene and Mu2 insertion site in the why1-3 allele. A fragment covering 380 bps Mu2 and 245 bps Why1 (dotted line) was deleted in the why1-3 allele. Exons are boxes and introns are lines. Translated regions are filled boxes. Triangles are Mu insertions in the why1-1 and why1-2 allele [33]. ATG: translation start codon, TGA: translation stop codon.