Changes in B Cell Populations and Merozoite Surface Protein-1-Specific Memory B Cell Responses after Prolonged Absence of Detectable P. falciparum Infection
Figure 4
The gating strategy for identifying the B cell subpopulations.
We first gated on the live (DAPI−) lymphocytes and then collected 10,000 events of CD19+. These were analyzed for expression of IgD, CD27, CD21, IgM and FcRL4. CD19+ lymphocytes were classified as naïve (IgD+CD27-) B cells, non class-switched (IgD+CD27+) MBCs, classical (IgD-CD27+) MBCs and double negative (IgD-CD27-) MBCs. MBC subtypes were further defined as follows: 1. Resting classical MBCs: CD19+IgD-CD27+CD21+. 2. Activated MBCs: CD19+IgD-CD27+CD21-. 3. Atypical MBCs: CD19+IgD-CD27-CD21- MBCs.