Biochemical Characterization of Paracoccidioides brasiliensis α-1,3-Glucanase Agn1p, and Its Functionality by Heterologous Expression in Schizosaccharomyces pombe
Figure 3
SDS-PAGE, and Western analysis of P. brasiliensis Agn1p.
Ni-NTA-purified Agn1p from cell lysates of E. coli transformed with of pQE-30Xa::AGN1 (Agn1p), and with the empty pQE-30Xa expression vector as negative control (NC) were separated by SDS-PAGE and stained with coomasie blue (A). The Ni-NTA-purified lysates were blotted on a nitrocellulose membrane and the His-tagged P. brasiliensis Agn1p (Agn1p) visualized using an anti RGS-His antibody (B). E stands for eluate, and NB for unbound material. MW: molecular weight marker. 6HP: 6xHis Ladder. Black arrow signals Agn1p position in both panels.