Molecular Fingerprint of High Fat Diet Induced Urinary Bladder Metabolic Dysfunction in a Rat Model
Figure 5
Validation of eNOS abundance and phosphorylation.
Western Blot analysis of eNOS showed decreased abundance of eNOS (A) and phospho eNOS 1177 (B) in HFD rats. (C) DAB staining revealed eNOS in both, the urothelium and detrusor. ENOS abundance (red) was quantified by confocal immunofluorescence of urothelium and detrusor (insets are 3× magnification from same image; nuclei are stained with DAPI (blue); detrusor express alpha actin (green); nc – negative control); star indicates cutting artifacts. (D) Analysis of confocal immunofluorescence showed higher eNOS expression in the urothelium (uroth) of HFD rats compared to their detrusor (det) while eNOS expression in CD rats did not differ between the urothelium and detrusor (n = 105 ROIs each column). Data are shown as mean ± SD. The dots represent the single values. Statistical evaluation by independent Student t-test, p<0.05 was considered as statistical significant.