The RhoGEF TEM4 Regulates Endothelial Cell Migration by Suppressing Actomyosin Contractility
Figure 4
RhoC is required to maintain protrusion dynamics of the leading edge.
(A) Frames of a time-lapse movie recording GFP-Lifeact to demonstrate organization of the actin filaments during migration of NS control (top row) or RhoC-depleted cells (bottom row). Green arrows indicate leading edge protrusion, with red arrows indicating edge retraction. Scale bar, 10 µm. (B) Kymography analysis of membrane protrusions of NS or RhoC-depleted cells. Sample kymographs and protrusion parameters of HUVECs depleted of RhoC or NS control. In each experimental group, 5–6 protrusions per cell in each of 4–6 cells were analyzed and data are mean ± s.e.m. (C) Time lapse montage of an area outlined in panel A showing a close up of actin filaments in protruding membranes of NS control (top row) or RhoC-depleted cells (bottom row). Asterisk marks an individual F-actin filament to highlight differential appearance between actin filaments in NS and RhoC-depleted cells.