Avian WNT4 in the Female Reproductive Tracts: Potential Role of Oviduct Development and Ovarian Carcinogenesis
Figure 1
Expression and localization of WNT4 in the chicken oviduct.
Both RT-PCR [A] and quantitative PCR [B] analyses were performed using cDNA templates from each segment of the chicken oviduct. These experiments were conducted in triplicate and normalized to control ACTB expression. [C] In situ hybridization analysis for cell-specific changes in expression of WNT4 in the each segment of the chicken oviduct. Both RT-PCR [D] and quantitative PCR [E] analyses were performed using cDNA templates from the magnum and the shell gland segment at 3 h and 20 h after ovulation. [F] In situ hybridization analysis for cell-specific changes in expression of WNT4 in the magnum and the shell gland at 3 h and 20 h after ovulation. Legend: ST, stromal cells; GE, glandular epithelium; LE, luminal epithelium. Scale bar represents 50 μm (the first and the third horizontal panels) and 20 μm (the second horizontal panel). The tissue samples were from oviducts of 1- to 2- year-old female laying hens (n = 5). The asterisks denote statistically significant differences (***P<0.001 and **P<0.01).