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Proteolytic Characteristics of Cathepsin D Related to the Recognition and Cleavage of Its Target Proteins

Figure 4

Screening of proteins that were insensitive to CD cleavage in A549-CR cells.

A, The expression of CD in A549 cells was evaluated by Western blotting. The control was transfected with empty vector. B, Comparison of the 2DE images obtained from lysates of A549-CR cells treated with/without CD. The insets represent the typical 2DE images showing spots that were sensitive or insensitive to CD cleavage.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0065733.g004