Evaluating Baculovirus as a Vector for Human Prostate Cancer Gene Therapy
Figure 3
BV transduction of cells growing in three dimensions.
(A) PC346C spheroids transduced with Bv-[CMV-EGFP] (or mock transduced) at 3 days post-infection. (B) PC346C tumours harvested from xenografted mice at 3 days post-infection with BV at 3×107 (one representative image shown) or 1×108 (two representative images shown) pfu per inoculation. Red fluorescence shows cell nuclei (Hoescht), while BV-driven EGFP expression is shown in green. Arrows indicate the site of intratumoural BV injection.