Differential Regulation of Extracellular Matrix and Soluble Fibulin-1 Levels by TGF-β1 in Airway Smooth Muscle Cells
Figure 5
FBLN-1 from a soluble source is incorporated into the ECM.
Cycloheximide treated and untreated human ASM cells were stimulated with or without 10 ng/ml TGF-β1 in quiescing medium or soluble FBLN-1 containing medium for 72 hours (n = 7). The incorporation of FBLN-1 into the matrix was detected by ECM ELISA and data were expressed as absorbance at 450 nm–570 nm. Data were expressed as mean ± SEM and analysed by one-way ANOVA with Bonferroni’s multiple comparison test, *P<0.05, ***P<0.001. V: vehicle; CHX: cycloheximide; S: soluble FBLN-1 containing medium; T: TGF-β1.