Zinc Finger Nuclease Mediated Knockout of ADP-Dependent Glucokinase in Cancer Cell Lines: Effects on Cell Survival and Mitochondrial Oxidative Metabolism
Figure 2
APDGK knockout in H460 cells using zinc-finger nucleases.
(A) Western blots of H460 cells after transfection with ADPGK ZFNs (clones ID5 and VD6), and ADPGK-null clones derived from these in a second round of ZFN transfection (IID10 and IIE5). (B) Genomic DNA copy number by qPCR for primer pairs CNV1-3. Results are plotted relative to WT DNA which has three ADPGK alleles. (C) Sequencing across the ZFN cut site shows deletions (−) and insertions (underlined) for the ADPGK alleles in H460 clones IID10 and IIE5.