Differential Effects of Activated Human Renal Epithelial Cells on T-Cell Migration
Figure 1
IFN-γ and TNF-α stimulation upregulates expression of activation markers CD40, HLA-I and HLA-DR by TECs.
Primary TECs were stimulated with IFN-γ (50 ng/ml)/TNF-α (20 ng/ml) for 24, 48 and 72 hours. (A) Surface staining was performed for the activation markers CD40, HLA-I and HLA-DR. One representative out of 10 experiments is shown. The upregulation of activation markers was observed after 24 h stimulation and still detectable after 72 h stimulation (data not shown). (B) Primary TECs were also stained for the costimulatory molecules CD80 and CD86. The dotted line represents unstained control, grey bar/line represents unstimulated situation and the dark bar/line represents IFN-γ/TNF-α stimulation (C) Quantification of flow cytometric results (mean ± SEM, n = 10) indicating the median fluorescence intensity of the activation markers CD40, HLA-I and HLA-II (n = 3). White bars represent unstained control, grey bars represent unstimulated condition and black bars represent IFN-γ/TNF-α stimulation *** Significant increase (P <0.001).