Skip to main content
Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

Development and Evaluation of a Pseudovirus-Luciferase Assay for Rapid and Quantitative Detection of Neutralizing Antibodies against Enterovirus 71

Figure 2

Comparison between CPE and PVLA measurements.

(A) Correlation analysis was performed for 180 double-positive EV71-NtAb samples tested by both the CPE and PVLA methods. The solid line represents the linear regression fitting of the data. The result demonstrated that there was a good relativity between the two assays (r = 0.91, p<0.0001). (B) A Bland-Altman difference plot of EV71-NtAb measurements was constructed using the results of the CPE and PVLA methods. The x-axis corresponds to the average (log10) concentration of EV71-NTAb determined by CPE or PVLA, and the y-axis is a measure of the difference between the concentrations as determined by CPE and PVLA. The solid line represents the mean value, while dashed lines represent the 95% confidence limits. The results indicated that the new method has a high-degree of agreement with the “gold standard” assay. Only four values fell outside ±2 standard deviations (−1.003 to 0.809log10).

Figure 2

doi: https://doi.org/10.1371/journal.pone.0064116.g002