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Tagging the Expressed Protein with 6 Histidines: Rapid Cloning of an Amplicon with Three Options

Figure 6

Diagrammatic representation of the cloning of a PCR amplified DNA segment into the three vectors.

The amplified product should contain the ATG. The blunt-end DNA segment can be cloned in all the three vectors.

Figure 6

doi: https://doi.org/10.1371/journal.pone.0063922.g006