Dynamic Regulation of Extracellular Signal-Regulated Kinase (ERK) by Protein Phosphatase 2A Regulatory Subunit B56γ1 in Nuclei Induces Cell Migration
Figure 6
IER3 knockdown decreases cell migration and increase ERK dephosphorylation.
(A) The effect of IER3 RNAi on migration of Calu1 cells. (B) The effect of RNAi on IER3 mRNA expression. (C) The effect of IER3 RNAi on the structure of actin cytoskeleton. The numbers of cells with cytoplasmic processes were counted under a fluorescence microscope. (D) The effect of IER3 RNAi on kinetics of pYERK and pTpYERK. Bars, 10 µm. Two way analysis of variance (A) or Student’s t-test (B, C, D) was used for statistical analysis.