Hypoxic Tumor Kinase Signaling Mediated by STAT5A in Development of Castration-Resistant Prostate Cancer
Figure 3
Kinase activity in androgen-deprivation therapy (ADT)-naïve xenografts versus ADT-naïve patient tumors.
An ADT-naïve kinase activity signature was defined as the 100 kinase peptide substrates with highest phosphorylation levels. For prostate carcinoma xenografts, the signature was represented by the mean of all ADT-naïve substrate phosphorylation levels. For prostate cancer patients, a top 100 signature was produced by calculating the mean of the three ADT-naïve (case 1, 2, and 3; Table 1) tumor phosphorylation levels. (A) Seventy-four of the 100 substrates were shared by the xenograft and the patients’ tumor ADT-naïve signatures. (B) Phosphorylated kinase peptide substrates shared by ADT-naïve prostate carcinoma xenografts (n = 3) and ADT-naïve tumors from prostate cancer patients (mean of case 1, 2, and 3). Listed are the substrates’ corresponding gene names, with start and end positions for the peptide sequence within the protein in brackets. (C) The exclusively phosphorylated kinase peptide substrates (n = 26) in the xenograft signature (left) and in the patient tumor signature (right).