Dynamic Nucleosome Organization at hox Promoters during Zebrafish Embryogenesis
Figure 6
Exogenous RA does not affect nucleosome organization at hox promoters.
(A–L) Average nucleosome density was calculated as in figure 1 for expressed (red line in panels A, B, D, E, G, H, J, K) and non-expressed (blue lines in panels A, C, D, F, G, I, J, L) promoters at 2 hpf (A–C), 4 hpf (D–F), 6 hpf (G–I) and 9 hpf (J–L). Nucleosome densities at induced and uninduced promoters were compared using a Wilcoxon Ranked Sum test and statistically significant differences (p<0.05) are indicated in green on the horizontal line in panels A, D, G and J. (M–P) Overlay of nucleosome profiles for expressed promoters in untreated (green line) and RA-treated (red line) embryos at 2 hpf (M), 4 hpf (N), 6 hpf (O) and 9 hpf (P).