Recombinant Adeno-Associated Virus Serotype 6 Efficiently Transduces Primary Human Melanocytes
Figure 3
Mutation of surface-exposed tyrosine residues on the rAAV6 capsid does not increase transduction of primary human melanocytes.
Melanocytes were transduced with wild type rAAV6, rAAV6 Y444F, rAAV6 Y731F, or the double mutant rAAV6 Y444F+Y731F at 105 vector genomes per cell and eGFP expression assessed by flow cytometry 48 hours post infection. Data points represent the average of technical replicates (n = 1−3) in two donors. Results are expressed relative to wild type.