Upregulation of miR-150* and miR-630 Induces Apoptosis in Pancreatic Cancer Cells by Targeting IGF-1R
Figure 4
Inhibitor of miR-150* blocked 3-Cl-AHPC-mediated decreased of c-Myb in PANC-1 cells.
(A) Inhibitor 2′-O-methylated miR-150* (OME-150) inhibited 3-Cl-AHPC mediated miR-150* expression in transiently transfected cells. (B and C) miR-150 inhibitor blocked the 3-Cl-AHPC mediated c-Myb degradation. Cells were treated with 1 µM 3-Cl-AHPC for 24 h. (D) Knock down of c-Myb expression in cells. (E) Knock down of c-Myb enhanced the apoptosis in cells. Cells stably transfected with three sh-Myb knockdown expression vectors and scrumble sequence control vector were exposed to 3-Cl-AHPC for 24 h. Induction of apoptosis was assessed using Annexin V-FITC labeling with propidium iodide (PI) staining in cells and the error bars represent the mean of three separate determinations ± the standard deviation (SD). All treated samples and sh-Myb are significantly different from control and sh-vector; • and ••, ** (<0.05 and <0.01 respectively) are significantly different by t-Test and • represented the comparison between 3-Cl-AHPC to OME-150*+3-Cl-AHPC treated samples.