Resveratrol Confers Protection against Rotenone-Induced Neurotoxicity by Modulating Myeloperoxidase Levels in Glial Cells
Figure 3
MPO-dependent increase of MPO levels are attenuated by resveratrol in rat primary microglia and astrocytes.
A. Rat primary microglia (PM, upper) and astrocytes (PA, lower) were pretreated with resveratrol (RESV), ethyl pyruvate (EtPy), or 15d-PGJ2 for 1 h, followed by incubation with 100 ng/ml MPO for 18 h. MPO levels were determined by Western blot analyses. B. Concentration-dependent effects of resveratrol were observed in MPO-treated primary microglia. C. Rat primary microglia were treated with 20 µM resveratrol and/or 100 ng/ml MPO at various time points. The cells were further incubated for 18 h and then MPO levels were examined by western blot analyses. The data shown are representative of at least three independent experiments.