BMP2 Genetically Engineered MSCs and EPCs Promote Vascularized Bone Regeneration in Rat Critical-Sized Calvarial Bone Defects
Figure 3
qRT-PCR analysis of osteoblast and endothelial marker genes.
MSCs, EPCs or MSCs and EPCs with or without BMP2 gene modification (1∶1) were plated in 24-well plate as a density of 2.25×104 cells/cm2 as described in Materials and Methods. These groups were MSCs alone (M), EPCs alone (E), MSCs +EPCs (M+E), BMP2 gene-modified MSCs (B2/M), BMP2 gene-modified EPCs (B2/E), EPC+BMP2 gene-modified MSCs (B2/M+E), MSC+BMP2 gene-modified EPCs (B2/E+M), and BMP2 gene-modified MSCs+BMP2 gene-modified EPCs (B2/(M+E)). The cells were induced with EGM/OS media for 14 days for qRT-PCR analysis. Data represent the mean+SD of n = 6 samples. (A) Osteoblast marker genes: OCN, Col I and BMP2. (B) Endothelial cell marker genes: VEGF, cdh5 and vWF. For A and B, ND: p>0.05, there is no significant difference between any two groups among the indicated ND groups. * p<0.05: between any two groups except the ND groups. # p<0.05: any ND groups vs. any other groups. ▾ p<0.05, any group in ND groups vs. any other groups.