Flow Cytometric Assessment of Erythrocyte Shape through Analysis of FSC Histograms: Use of Kurtosis and Implications for Longitudinal Evaluation
Figure 1
Selected FSC histograms illustrating bimodal and monomodal distributions.
Selected FSC histograms illustrating bimodal and monomodal distributions. x-axis displays the channels used for analysis, y-axis gives the number of events for each channel. Dashed lines indicate channel 101 chosen for calculation of SphI. The median value for the respective part of the distribution is marked by short lines. For subject 11 (Full, IgG2b, d7), kurtosis = −1.21, PCD = −0.15, SphI = 2.47 (median 1 = 148, median 2 = 60). For subject 16 (Full, IgG2b, d3), kurtosis = −0.36, PCD = −0.27, SphI = 1.70 (median 1 = 129, median 2 = 76).