Development of the Mouse Dermal Adipose Layer Occurs Independently of Subcutaneous Adipose Tissue and Is Marked by Restricted Early Expression of FABP4
Figure 6
Trichorhinophalangeal syndrome I (TRPS1) expression is restricted to upper dermal cells at embryonic day 17 (e17), e18 and e19 in murine dermis.
a), DNA was counterstained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bar = 30 µm. b) The relative mRNA expression for TRPS1 was analysed from samples at the e17 (17), e18 (18) and e19 (19) time-points from upper (Area “1”, blue) and lower (Area “2”, red) dermis. The baseline (1-fold change) was established for the e19 upper dermis sample (underlined on the figure) and the mRNA levels of the other samples are shown relative to this. TRPS1 mRNA is up-regulated in upper dermis (Area “1”) compared with lower dermis (Area “2”) at all analysed time-points. Data were obtained from triplicate biological replicates. P value refers to the comparison with “19 1” sample (*P≤0.05, **P≤0.01, ***P≤0.001).