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T Cells Contribute to Stroke-Induced Lymphopenia in Rats

Figure 2

Cortical infarction and inflammation in WT and nude rats after stroke.

A. Representative infarctions in the cortex are shown. Animals were euthanized 3 d after stroke. Brains were collected, coronally sectioned into 5 slices and stained with a 2% TTC solution. B. Infarct regions were measured and normalized to the contralateral, non-ischemic cortex, and expressed as percentage. The bar graphs represent average infarct sizes (n = 7–9/group). C. CD 68-positive macrophage staining in the ischemic brain. Ischemic brains were collected 72 h post-stroke and immunostained with CD 68 antibodies (green), and counterstained with DAPI (blue). CD 68 positive cells were counted from 3 sections of each animal. Representative images of CD68 positive macrophages are shown. The statistical results suggest no difference in the CD 68 positive macrophage numbers between WT and nude rats. N = 4–5/group. Scale Bar: 50 μm. D. Ischemic brain cortices were collected 3 d after stroke and total mononuclear cells were extracted for FACS analyses. Data from the non-ischemic hemispheres were used as controls. Gating strategy for T cells and subpopulations are shown on the upper panel. In the ischemic brain, numbers of T cells and CD4+ T cells, as well as CD8+ T cells were significantly increased compared with those in the control hemisphere. Note that nude rats, in principle, have no T cells, including CD4+ T cells, CD8+ T cells. * vs. corresponding controls (the contralateral, non-ischemic hemisphere), P<0.05, n = 4.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0059602.g002