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Meckelin 3 Is Necessary for Photoreceptor Outer Segment Development in Rat Meckel Syndrome

Figure 4

Loss of photoreceptors between P10 and P21 in MKS3 mutant.

To determine the effect of Mks3 mutation in photoreceptors at P10 and P21, sections were immunolabeled for rhodopsin (A–D), L/M opsin (E–H), or S opsin (I–L). At P10, rhodopsin-labeled cells were easily detected in seemingly similar numbers in the WT and mutant retinae (A, B). Both L/M opsin- and S opsin-labeled cells were not abundant at P10 in either the WT or mutant retinae (E, F and I, J). By P21, rhodopsin was highly abundant in the photoreceptor outer segments of WT retinae, but was present in the cell bodies of the photoreceptors of mutant retinae (C, D). Similarly, outer segments labeled for L/M opsin were easily detected in WT photoreceptors, but staining was localized to the cell body of mutant photoreceptors (G, H). S opsin-positive cells were detectable in WT photoreceptor outer segments, but very few positive cells were detected in mutant retinae (arrow; K, L). DAPI label of the section is shown in a small strip on the right-hand side of each picture to indicate placement of the retinal cell layers (A–L). Quantitatively, the number of CRX (+) cells was very similar in WT and mutant retinae at P10 (M). However, there was a large drop in the number of rods and a smaller, but statistically significant decrease in the number of L/M cones was found in the fundus of the mutant retinae in comparison to the WT (N). GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; OS, outer segment; WT, wild type; M, mutant. ** unpaired t-test p>0.005. * unpaired t-test p>0.05. Scale bar: (A) 50 µm.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0059306.g004