In Vivo Detection of Human TRPV6-Rich Tumors with Anti-Cancer Peptides Derived from Soricidin
Figure 4
In vivo optical imaging of mice bearing SKOV-3 xenograft tumors after i.p. administration of 100 µg of SOR-C27 tagged with Cy5.5.
Dorsal whole-body images at indicated time points after injection of either SOR-C27-Cy5.5 (upper panels of A) or SOR-C27-Cy5.5 competitively blocked by 100 fold excess of SOR-C27 (lower panels of A). Arrows indicate the location of the solid subcutaneous tumor in the flank of the animal. Graph illustrating the changes in average fluorescence concentration in the tumor at indicated times after injection of SOR-C27-Cy5.5 or SOR-C27-Cy5.5 competitively blocked by SOR-C27 (B). Ex vivo optical images of mouse organs 24 hours post-injection of SOR-C27-Cy5.5 alone (left panel of C) or SOR-C27-Cy5.5 competitively blocked by SOR-C27 (right panel of C). Graph illustrating the average fluorescence concentration imaged ex vivo in various tissues 24 hours post-injection of SOR-C27-Cy5.5 or SOR-C27-Cy5.5 competitively blocked by SOR-C27 (D). *Indicates significant difference between SOR-C27-Cy5.5 and SOR-C27-Cy5.5 competitively blocked SOR-C27-Cy5.5 (p<0.05). In B and D, data are expressed as mean ± SEM for n = 3 animals.