FISHIS: Fluorescence In Situ Hybridization in Suspension and Chromosome Flow Sorting Made Easy
Figure 1
FISHIS of pasta wheat cv Creso chromosome suspensions.
a) Chromosome suspensions hybridized with (GAA)7-FITC; b) flow-sorted chromosomes 3B, 4B and 5B following hybridization with (AG)12-Cy3; c) (GAA)7-FITC and (AAT)7-Cy3 dual labelled chromosomes and nuclei; d) pasta wheat chromosomes and a nucleus after (GAA)7-FITC and (AAC)5-Cy3 dual labeling. Bar = 10 µm.