Structure-Guided Systems-Level Engineering of Oxidation-Prone Methionine Residues in Catalytic Domain of an Alkaline α-Amylase from Alkalimonas amylolytica for Significant Improvement of Both Oxidative Stability and Catalytic Efficiency
Figure 6
Local hydrogen bonding network of the five-site mutants.
The active site is shown in a green CPK (Corey-Pauling-Koltun) representation. The yellow “ball and stick” indicate the conserved regions. The blue “stick” indicates the mutation site. The purple “stick” is the residue that forms new hydrogen bonding after mutation. The black “dotted line” is the hydrogen bonding. The α helices and β sheets are shown in red and cyan, respectively. A: M145A-214A-229A-247L-317I; B: M145I-214A-229T-247T-317I; C: M145A-214A-229T-247L-317I; D: M145A-214A-229T-247T-317I.