Maternal Transmission of a Humanised Igf2r Allele Results in an Igf2 Dependent Hypomorphic and Non-Viable Growth Phenotype
Figure 4
Rescue of Igf2r+m/HUpmaternal transmission growth phenotype by loss of function of Igf2 and gain of function of Igf2r.
(A) Maternal transmission to generate Igf2rHUm/+p was combined with paternal transmission of the paternal null allele of Igf2+m/−p to generate combined heterozygotes (Igf2rHUm/+p, Igf2+m/−p). Post-natal growth curves show that maternal transmission of the maternal allele did not alter growth rates when lethality was rescued by loss of Igf2 (Table 1). (B) Lethality of Igf2rHUm/+p was rescued by paternal expression of Igf2r murine allele (R2Δ+m/−p, see Table 1) and subsequent post-natal growth kinetics appeared similar.