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Connexin 30 Expression and Frequency of Connexin Heterogeneity in Astrocyte Gap Junction Plaques Increase with Age in the Rat Retina

Figure 5

Six Marker Immunohistochemistry Connexins on Astrocytes Predominantly in the Parenchyma in the Retina of a Middle Aged Rat.

Cx26 (blue, A), Cx30 (red, B), Cx43 (green, C), Cx45 (pale brown, D) were colocalised (E) on GFAP positive-astrocytes (pale purple, F). Connexin heterogeneity in gap junction plaques are indicated as follows: Cx26+/Cx43+ (large arrows), Cx26+/Cx45+ (small arrows), Cx26+/Cx30+/Cx43+ (curved arrows), Cx26+/Cx43+/Cx45+ (arrowheads). Scale bar, 50 µm (A–F).

Figure 5

doi: https://doi.org/10.1371/journal.pone.0057038.g005