Induction of Apoptosis in Human Breast Cancer Cells via Caspase Pathway by Vernodalin Isolated from Centratherum anthelminticum (L.) Seeds
Figure 14
Vernodalin reduces expression of pro-survival molecules.
MCF-7 and MDA-MB-231 cells were treated with control DMSO, standard drug doxorubicin (12.5 µg/ml) or various concentrations of vernodalin (3.125, 6.25, 12.5 µg/ml). Western blot showing the expression levels of the pro-survival molecules Bcl-2 and Bcl-xL in untreated and treated breast cancer cells. β-actin served as a loading control. Decreased Bcl-2 and Bcl-xL protein levels were observed upon doxorubicin or vernodalin treatment. Data were representative of at least two similar experiments.