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Using Haloarcula marismortui Bacteriorhodopsin as a Fusion Tag for Enhancing and Visible Expression of Integral Membrane Proteins in Escherichia coli

Figure 4

Characterization of BRs.

(A) The absorbance spectrum of BRs. The UV-Vis scan was plotted from 250 to 750 nm, and the absorbance peak is at 552 nm. (B) The purified HmBRI/D94N, which shows a single band on SDS-PAGE, is a purple solution. The SEC profile (C) and crystal (D) of purified HmBRI/D94N and the SEC profile (E) and crystal (F) of HwBR are shown. (G) The X-ray diffraction pattern and (H) The structural model of HmBRI/D94N.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0056363.g004