Leishmania major Methionine Sulfoxide Reductase A Is Required for Resistance to Oxidative Stress and Efficient Replication in Macrophages
Figure 3
LC-MS quantification of intracellular MetO and Met levels in L. major grown in varying concentrations of Met and MetO.
A. Abundance of free MetO (comprising a doublet of Met-S-O/Met-R-O isomers) and Met in total extracts of wild type (upper panel) and ΔmsrA (lower panel) promastigotes cultivated in media containing 80 µM MetO and 20 µM Met. B. Ratio of MetO to Met levels in L. major wild type (black columns) and ΔmsrA (white columns) grown in varying concentrations of MetO.