SHP-1 Regulation of Mast Cell Function in Allergic Inflammation and Anaphylaxis
Figure 2
SHP-1 and mast cell regulation of LPS induced airway inflammation.
Mice were stimulated with LPS (100 ng) or PBS by intranasal (i.n.) instillation. (A, B) One day later, BAL total and differential cell counts were determined and compared among WT, mev/+, KitW-sh, and mev/+/KitW-sh mice treated with LPS or PBS. KitW-sh mice received adaptively transferred BMMC from either WT or mev/mev mice and stimulated with LPS were also shown (*P < 0.05; **P < 0.01). (C). Mast cell reconstitution in KitW-sh mice. Toluidine blue staining (40x) and mast cell counts in the spleen sections from KitW-sh mice that received either WT or mev/mev BMMC as compared to WT and KitW-sh controls. (D). Lung histology. H&E stained lung sections from WT, mev/+, KitW-sh, and mev/+/KitW-sh mice with or without LPS stimulation. Shown are representative slides of at least 5 individual samples for each group (Magnification 20x).