The Quiescin Sulfhydryl Oxidase (hQSOX1b) Tunes the Expression of Resistin-Like Molecule Alpha (RELM-α or mFIZZ1) in a Wheat Germ Cell-Free Extract
Figure 6
mFIZZ1′ and mFIZZ1 decreased the IL-13 and IL-5 secretion of splenocytes.
Splenocytes were cultured at 200,000 cells/well and activated under Th2 permissive conditions for 4 days. Recombinant mFIZZ1′ and mFIZZ1 expressed with or without hQSOX1b were used at 200 ng/ml. rRa is the bacterial recombinant FIZZ1 (200 ng/ml) from (Peprotech) and PBS is the control. (A) Recombinant mFIZZ1′ and mFIZZ1 co-expressed with hQSOX1b significantly decreased the IL-5 secretion compared to the proteins expressed alone. (B) Recombinant mFIZZ1′ and mFIZZ1 co-expressed with hQSOX1b decreased the IL-13 secretion compared to the proteins expressed alone. ***P<0.001; **P<0.01; *P<0.05; #P = 0.07. Results are representative of three independent experiments for mFIZZ1′ and two independent experiments for mFIZZ1, and statistical analysis was performed by two-way ANOVA.