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Localization of Lipid Raft Proteins to the Plasma Membrane Is a Major Function of the Phospholipid Transfer Protein Sec14

Figure 1

The Sec14 ^G266D protein can provide the essential function of Sec14.

Serial dilutions of cells cultured at 25°C and then plated and grown at 25°C and 37°C for 3 days. A, wild type, sec14^ts and sec14^ts rpn4Δ. B, The sec14^ts strain transformed with either empty vector, a plasmid carried at low copy (ARS/CEN) containing wild type Sec14, or a high copy (2 μ) plasmid containing Sec14^G266D.

doi: https://doi.org/10.1371/journal.pone.0055388.g001