Overexpression of Wild-Type but Not C134W Mutant FOXL2 Enhances GnRH-Induced Cell Apoptosis by Increasing GnRH Receptor Expression in Human Granulosa Cell Tumors
Figure 4
GnRH-induced apoptosis is enhanced by wild-type but not C134W mutant FOXL2.
A, KGN cells were transfected with empty pcDNA3 plasmid (Ctrl), wild-type FOXL2 (WT) or C134W mutant FOXL2 (C134W) for 24 hr and then treated with GnRH-I or GnRH-II (10−7 M) for 96 hr. Cell viability was examined by MTT assay. B, KGN cells were transfected as described in (A) and then treated with GnRH-I (G-I) or GnRH-II (G-II) (10−7 M) for 72 hr. Cleaved caspase-3 protein levels were measured by Western blot. Results are expressed as mean ± SEM of at least three independent experiments. Values without a common letter are significantly different (p<0.05).