Transcriptome Analysis of Epigenetically Modulated Genome Indicates Signature Genes in Manifestation of Type 1 Diabetes and Its Prevention in NOD Mice
Figure 6
Modulation of genes implicated in various cellular functions by TSA treatment.
(A) Total RNA was derived from splenocytes of diabetic and cured mice as indicated in Figure 4 & 5, and used for analyzing the expression of anti-oxidant genes and additional genes by qRT-PCR. (B) Total RNA derived from pancreata was probed similarly for the expression of indicated genes. (C) Splenic T lymphocytes from diabetic and cured mice were stimulated with immobilized anti-CD3, RNA extracted and analyzed for Mif expression. Steady state level expression of Mif was also analyzed in pancreata by qRT-PCR. Fold-increase in Mif gene expression is indicated. Each data point represents mean +/− SD of triplicate determinations. P values are given. NS indicates not significant. SC, splenocytes.